MODULATION OF INTERLEUKIN-1Β (IL-1Β ), TUMOR NECROSIS FACTOR–Α (TNF-Α) AND INTERLEUKIN-10 (IL-10 ) GENES EXPRESSION FOLLOWING CONCURRENT TRAINING IN WOMEN WITH TYPE2 DIABETES

Background: The purpose of this study was to investigate the effect of concurrent training on pre-inflammatory (IL-1β, TNF-α) and anti-inflammatory (IL-10) cytokines gene expression in women with type2 diabetes. Methods: 18-patients (age30-38, >130 glycemic index) were selected, randomly, and divided into control (n=6) the concurrent training(diabetes, n=6) and the concurrent training (healthy, n=6)  groups. The concurrent training protocol consisted of 3 sessions resistance training per week, 8sets with 80% one maximum repetition( and the endurance training preformed with 30- minutes running (3sets×10 minutes) on a treadmill with 70-80 maximum heart rate(70-80 MHR), immediately. The leukocyte’s IL-1β, TNF-α and IL-10 genes expression determined by the Real time-PCR technique. The quantitative expression of the cytokines gene was calculated using the 2-ΔΔ CT method. The between- groups differences in variables were determined by independent t-test (permutation test) through REST software and independent one-way ANOVA. Results: The results showed that IL-1β, TNF-α mRNA genes expression reduced significantly after the concurrent training in both the training groups in comparison to the control group (P<0.05). In addition, the results also showed that IL-10 mRNA gene expression was not expressed in leukocytes after the concurrent training in both training groups in comparison to the control group (P>0.05). Conclusion: In conclusion, the results suggest that the concurrent training modulate IL-1β and TNF-α mRNA genes expression significantly in diabetic women but could not change IL-10 genes expression. This type of exercise training seems to be more effective in reducing the expression of pro-inflammatory cytokines than in enhancing anti-inflammatory cytokines.